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Friday, June 23rd, 2017
Table of Contents

1 Introduction
5 PMID
 [F] Diseases Research  / PubMed Research Articles  /
Activation of Nrf2/HO-1 Pathway by Glycogen Synthase Kinase-3β Inhibition Attenuates Renal Ischemia/Reperfusion Injury in Diabetic Rats.

PubMed

 

Resource

Kidney & blood pressure research 2017 18; 42(2)

Authors

Shen X1; Hu B2; Xu G3; Chen F4; Ma R5; Zhang N6; Liu J7; Ma X8; Zhu J9; Wu Y10; Shen R11;

Author Information
  • 1Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 2Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 3Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, China.
  • 4Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 5Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 6Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 7Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 8Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 9Department of Pathology and Pathophysiology, Provincial Key Discipline of Pharmacology, Jiaxing University Medical College, Jiaxing, China.
  • 10Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.
  • 11Department of Chemical Pathology, Urinary Surgery, Nephrology, Gynecology and Intensive Care Unit, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University Affiliated TCM Hospital, Jiaxing, China.

Abstract

BACKGROUND/AIMS: Diabetes mellitus can exacerbate renal ischemia-reperfusion (I/R) injury (RI/RI). The aim of the present study was to evaluate the protective effect of GSK-3β inhibition (TDZD-8) on I/R-induced renal injury through the Nrf2/HO-1 pathway in a streptozocin (STZ)-induced diabetic rat model.

METHODS: STZ-induced diabetic rats preconditioned with TDZD-8 and ZnPP were subjected to renal I/R. The extent of renal morphologic lesions. Renal function was assessed from blood urea nitrogen (BUN) and serum creatinine (Scr), as determined utlizing commercial kits. Oxidative stress and inflammatory activity in the kidney tissue was estimated from levels of malondialdehyde (MDA), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and nitric oxide (NO), as well as the activities of superoxide dismutase (SOD) and glutathione (GSH) using qRT-PCR and ELISA. The expressions of Nrf2, HO-1, Bcl-2 and NF-κB in the renal tissue were measured by qRT-PCR and western blotting.

RESULTS: I/R-induced renal inflammation was reduced significantly by TDZD-8 pretreatment. Preconditioning with TDZD-8 suppressed NF-κB expression and enhanced Bcl-2 expression in the renal tissue. The upregulated level of malondialdehyde (MDA), and reduced activities of superoxide dismutase (SOD) and glutathione (GSH) in I/R-shocked rats were markedly restored by TDZD-8 pretreatment. Furthermore, pretreatment with TDZD-8 enhanced activation of the Nrf2/HO-1 pathway in the renal tissue of diabetic RI/RI rats.

CONCLUSION: These findings suggest that preconditioning with TDZD-8 may protect the kidney from I/R-induced damage via the activation of the Nrf2/HO-1 pathway in STZ-induced diabetic rats. Further detailed studies are needed to further clarify the underlying mechanisms.

© 2017 The Author(s). Published by S. Karger AG, Basel.

PMID

28624830

Others

Publication Type: Journal Article


This article is licensed under the the National Library of Medicine License. It uses material from the PubMed National Library of Medicine Data.


Last Modified:   2016-03-27


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